Part:BBa_K2668020:Design

Sirius: CBM3a - mRFP1 fusion

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1272
  Illegal AgeI site found at 1384
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The part BBa_K2668020 named Sirus comes from the iGEM team Toulouse-INSA-UPS 2018 Cerberus Project. It was designed to investigate the binding capability of CBM3a to cellulose.

Source

The part was obtained by synthesis from IDT. The CBM3a domain is a corrected version of the Part:BBa_K1321014. The mRFP1 is a corrected version of the Part:BBa_E1010.